Agarose–starch gel electrophoresis of rat serum lipoproteins

Microscope slide electrophoresis of serum lipoproteins in agarose gel.

An ultramicro electrophoretic technique for the separation of serum lipoproteins is described using agarose gel on microscope slides.The interaction between the agarose gel and lipoproteins has been reduced and the separation between beta- and pre-beta-lipoproteins has been improved over existing methods. A semiquantitative assay of the lipoproteins can be done in a densitometer. It is suggeste...

Starch-gel electrophoresis of rat-serum proteins.

In 1955, Smithies (1) described a procedure for the separation of human serum proteins by horizontal zone electrophoresis in starch gels. The separation of zones was improved by a later modification to a vertical electrophoresis in which the sample was inserted in the gel, free from supporting medium (2). With these methods, it has been demonstrated that many more protein fractions can be separ...

Gradient gel electrophoresis of human plasma lipoproteins.

A rapid and simple method for separating and identifying plasma lipoproteins is described. The technique utilizes the “molecular sieving” properties of continuous concave gradient electrophoresis on a slab of polyacrylamide (4 to 26%) gel. Gradient gel electrophoresis is particularly suitable for separating lipoproteins according to their molecular weight. The technique can be used to demonstra...

Thin-layer electrophoresis of serum lipoproteins.

A method is described for semiquantitative study of serum lipoproteins, with particular reference to the very low density fractions involved in transport of triglycerides. Serum samples were subjected to electrophoresis in thin layers of starch granules; lipids were extracted from starch segments and subjected to thin-layer chromatography; lipid fractions were recovered from the adsorbent and q...

Behavior of the Serum proteins in starch-agar-gel electrophoresis